CRISPR–Cas systems transcriptionally tune diverse innate defences using CRISPR RNA-like guides to balance antiviral protection with fitness, and hyperactivate these defences when compromised, establishing a layered bacterial ‘immunity guard’ network.
Bacterial strains and growth conditions
The bacterial strains used in this study are listed in Supplementary Table 3. The E. coli DH5 strain was used for plasmid construction, and the E. coli BL21 (… [+27331 chars]
The bacterial strains used in this study are listed in Supplementary Table 3. The E. coli DH5 strain was used for plasmid construction, and the E. coli BL21 (… [+27331 chars]