High-resolution in situ cryo-electron tomography in Schizosaccharomyces pombe identifies SNOR protein, which binds to ribosomes during dormancy induced by glucose depletion, priming them for rapid reactivation of protein synthesis upon glucose repletion.
Sample preparation for cryo-ET
Cryo-ET samples were prepared using 200-mesh R2/2 copper grids (Quantifoil) plasma-cleaned for 30s in 75% argon/25% oxygen using a 1070 plasma cleaner (Fischione). S. … [+29993 chars]
Cryo-ET samples were prepared using 200-mesh R2/2 copper grids (Quantifoil) plasma-cleaned for 30s in 75% argon/25% oxygen using a 1070 plasma cleaner (Fischione). S. … [+29993 chars]