Cas12a2 enables RNA-triggered, sequence-specific killing of eukaryotic cells via widespread DNA shredding, allowing selective elimination of cells on the basis of gene expression, including virus-infected or mutation-bearing cells.
Phylogenetic analysis
The amino acid sequence of GeCas12a2 was aligned with other Cas12a2 nuclease sequences15,59 using Clustal Omega60. The alignment was trimmed with ClipKIT61 and used to reconstr… [+35661 chars]
The amino acid sequence of GeCas12a2 was aligned with other Cas12a2 nuclease sequences15,59 using Clustal Omega60. The alignment was trimmed with ClipKIT61 and used to reconstr… [+35661 chars]