HIV-1 capsid nuclear import at the nuclear pore complex is a bottleneck to resting T cell infection, but HIV-1 overcomes this by triggering receptor-mediated signalling during cell–cell spread to drive nuclear import and licence infection.
Cells
Peripheral blood mononuclear cells (PBMCs) were isolated from leukocyte cones from healthy donors (NHS Blood and Transplant Services) by density centrifugation using Lympho 24+ Spin medium (Pl… [+36719 chars]
Peripheral blood mononuclear cells (PBMCs) were isolated from leukocyte cones from healthy donors (NHS Blood and Transplant Services) by density centrifugation using Lympho 24+ Spin medium (Pl… [+36719 chars]