Prime assembly enables RNA-guided integration of medium-to-large DNA sequences in human cells without requiring double-strand breaks or cell cycle progression, and supports exon recoding, transgene insertion and megabase-scale rearrangements.
Cell culture and nucleofection
K562 (CCL-243) and Jurkat (TIB-152) cells were obtained from American Type Culture Collection (ATCC) and cultured at 37°C under 5% CO2 in RPMI media (ThermoFisher Scie… [+35009 chars]
K562 (CCL-243) and Jurkat (TIB-152) cells were obtained from American Type Culture Collection (ATCC) and cultured at 37°C under 5% CO2 in RPMI media (ThermoFisher Scie… [+35009 chars]